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1pxw

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Revision as of 02:37, 3 May 2008 by OCA (Talk | contribs)
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Template:STRUCTURE 1pxw

Crystal structure of L7Ae sRNP core protein from Pyrococcus abyssii


Overview

The L7Ae sRNP core protein from Pyrococcus abyssii was crystallized using the sitting-drop vapour-diffusion method. Crystals were obtained in the presence of MgCl(2), PEG 2000 MME and acetate buffer at pH 4.0. A native data set has been collected at 2.9 A resolution using a rotating-anode generator at room temperature. Crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 70.7, b = 112.9, c = 34.8 A. There are two monomers of MW 14 200 Da per asymmetric unit and the packing density V(M) is 2.45 A(3) Da(-1). A molecular-replacement analysis gave solutions for the rotation and translation functions.

About this Structure

1PXW is a Single protein structure of sequence from Pyrococcus abyssi. Full crystallographic information is available from OCA.

Reference

Purification, crystallization and preliminary X-ray diffraction data of L7Ae sRNP core protein from Pyrococcus abyssii., Charron C, Manival X, Charpentier B, Branlant C, Aubry A, Acta Crystallogr D Biol Crystallogr. 2004 Jan;60(Pt 1):122-4. Epub 2003, Dec 18. PMID:14684904 Page seeded by OCA on Sat May 3 05:37:31 2008

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