1jyh

Structural highlights

Function

SBMC_ECOLI Inhibits the supercoiling activity of DNA gyrase. Acts by inhibiting DNA gyrase at an early step, prior to (or at the step of) binding of DNA by the gyrase. It protects cells against toxins that target DNA gyrase, by inhibiting activity of these toxins and reducing the formation of lethal double-strand breaks in the cell. Protects cells against the natural plasmid-encoded toxins microcin B17 (MccB17) and CcdB, and synthetic quinolones. Can also protect cells against alkylating agents that act independently of DNA gyrase, suggesting a more general role in protecting cells against DNA damage.^{[1] [2] [3] [4]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Chatterji M, Nagaraja V. GyrI: a counter-defensive strategy against proteinaceous inhibitors of DNA gyrase. EMBO Rep. 2002 Mar;3(3):261-7. Epub 2002 Feb 15. PMID:11850398 doi:http://dx.doi.org/10.1093/embo-reports/kvf038
2. ↑ Chatterji M, Sengupta S, Nagaraja V. Chromosomally encoded gyrase inhibitor GyrI protects Escherichia coli against DNA-damaging agents. Arch Microbiol. 2003 Nov;180(5):339-46. Epub 2003 Sep 11. PMID:13680098 doi:http://dx.doi.org/10.1007/s00203-003-0598-4
3. ↑ Baquero MR, Bouzon M, Varea J, Moreno F. sbmC, a stationary-phase induced SOS Escherichia coli gene, whose product protects cells from the DNA replication inhibitor microcin B17. Mol Microbiol. 1995 Oct;18(2):301-11. PMID:8709849
4. ↑ Nakanishi A, Oshida T, Matsushita T, Imajoh-Ohmi S, Ohnuki T. Identification of DNA gyrase inhibitor (GyrI) in Escherichia coli. J Biol Chem. 1998 Jan 23;273(4):1933-8. PMID:9442027