1oni

Structural highlights

Function

RIDA_HUMAN Catalyzes the hydrolytic deamination of enamine/imine intermediates that form during the course of normal metabolism. May facilitate the release of ammonia from these potentially toxic reactive metabolites, reducing their impact on cellular components. It may act on enamine/imine intermediates formed by several types of pyridoxal-5'-phosphate-dependent dehydratases including L-threonine dehydratase.^{[1] [2]} Also promotes endoribonucleolytic cleavage of some transcripts by promoting recruitment of the ribonuclease P/MRP complex (PubMed:8973653, PubMed:30930054). Acts by bridging YTHDF2 and the ribonuclease P/MRP complex (PubMed:30930054). RIDA/HRSP12 binds to N6-methyladenosine (m6A)-containing mRNAs containing a 5'-GGUUC-3' motif: cooperative binding of RIDA/HRSP12 and YTHDF2 to such transcripts lead to recruitment of the ribonuclease P/MRP complex and subsequent endoribonucleolytic cleavage (PubMed:30930054).^{[3] [4]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

References

1. ↑ Lambrecht JA, Browne BA, Downs DM. Members of the YjgF/YER057c/UK114 family of proteins inhibit phosphoribosylamine synthesis in vitro. J Biol Chem. 2010 Nov 5;285(45):34401-7. PMID:20817725 doi:10.1074/jbc.M110.160515
2. ↑ Lambrecht JA, Flynn JM, Downs DM. Conserved YjgF protein family deaminates reactive enamine/imine intermediates of pyridoxal 5'-phosphate (PLP)-dependent enzyme reactions. J Biol Chem. 2012 Jan 27;287(5):3454-61. doi: 10.1074/jbc.M111.304477. Epub 2011 , Nov 17. PMID:22094463 doi:http://dx.doi.org/10.1074/jbc.M111.304477
3. ↑ Park OH, Ha H, Lee Y, Boo SH, Kwon DH, Song HK, Kim YK. Endoribonucleolytic Cleavage of m(6)A-Containing RNAs by RNase P/MRP Complex. Mol Cell. 2019 May 2;74(3):494-507.e8. PMID:30930054 doi:10.1016/j.molcel.2019.02.034
4. ↑ Schmiedeknecht G, Kerkhoff C, Orsó E, Stöhr J, Aslanidis C, Nagy GM, Knuechel R, Schmitz G. Isolation and characterization of a 14.5-kDa trichloroacetic-acid-soluble translational inhibitor protein from human monocytes that is upregulated upon cellular differentiation. Eur J Biochem. 1996 Dec 1;242(2):339-51. PMID:8973653 doi:10.1111/j.1432-1033.1996.0339r.x