1qdp
From Proteopedia
SOLUTION STRUCTURE OF ROBUSTOXIN, THE LETHAL NEUROTOXIN FROM THE FUNNEL WEB SPIDER ATRAX ROBUSTUS, NMR, 20 STRUCTURES
Overview
The solution structure of robustoxin, the lethal neurotoxin from the Sydney funnel-web spider Atrax robustus, has been determined from 2D 1H NMR data. Robustoxin is a polypeptide of 42 residues cross-linked by four disulphide bonds, the connectivities of which were determined from NMR data and trial structure calculations to be 1-15, 8-20, 14-31 and 16-42 (a 1-4/2-6/3-7/5-8 pattern). The structure consists of a small three-stranded, anti-parallel beta-sheet and a series of interlocking gamma-turns at the C-terminus. It also contains a cystine knot, thus placing it in the inhibitor cystine knot motif family of structures, which includes the omega-conotoxins and a number of plant and animal toxins and protease inhibitors. Robustoxin contains three distinct charged patches on its surface, and an extended loop that includes several aromatic and non-polar residues. Both of these structural features may play a role in its binding to the voltage-gated sodium channel.
About this Structure
1QDP is a Single protein structure of sequence from Atrax robustus. Full crystallographic information is available from OCA.
Reference
Solution structure of robustoxin, the lethal neurotoxin from the funnel-web spider Atrax robustus., Pallaghy PK, Alewood D, Alewood PF, Norton RS, FEBS Lett. 1997 Dec 15;419(2-3):191-6. PMID:9428632 Page seeded by OCA on Sat May 3 06:10:06 2008
