1soh

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The structure of human apolipoprotein C-II in dodecyl phosphocholine

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The structure of human apolipoprotein C-II (apoC-II) in the presence of, dodecyl phosphocholine (DPC) micelles has been investigated by NMR, spectroscopy. The resulting structural information is compared to that, available for apoC-II in the presence of sodium dodecyl sulfate, revealing, a high level of overall similarity but several significant differences., These findings further our understandings of the structural basis for, apoC-II function. The interactions of the protein with the detergent, micelle are probed using intermolecular nuclear Overhauser effects (NOEs), and paramagnetic agents. These interactions are seen across almost the, full length of apoC-II and show the periodicity expected for an, amphipathic helix interacting with the amphipathic surface of the DPC, micelle. Furthermore, we observe specific contacts between lysine residues, of apoC-II and protons near the phosphate group of DPC, consistent with, the predictions of the so-called "snorkel hypothesis" of the structural, basis for the apolipoprotein/lipid interaction (Segrest, J. P., Jackson, R. L., Morrisett, J. D., and Gotto, A. M., Jr. (1974) A molecular theory, of lipid-protein interactions in the plasma lipoproteins, FEBS Lett 38, 247-258.). These findings offer the most detailed structural information, available for the interaction between an apolipoprotein and the, phospholipids of the lipoprotein surface and provide the first direct, structural support for the snorkel hypothesis.

Disease

Known disease associated with this structure: Hyperlipoproteinemia, type Ib OMIM:[608083]

About this Structure

1SOH is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

The structure and interactions of human apolipoprotein C-II in dodecyl phosphocholine., MacRaild CA, Howlett GJ, Gooley PR, Biochemistry. 2004 Jun 29;43(25):8084-93. PMID:15209504

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