1spj
From Proteopedia
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STRUCTURE OF MATURE HUMAN TISSUE KALLIKREIN (HUMAN KALLIKREIN 1 OR KLK1) AT 1.70 ANGSTROM RESOLUTION WITH VACANT ACTIVE SITE
Contents |
Overview
Human kallikreins are serine proteases that comprise a recently identified, large and closely related 15-member family. The kallikreins include both, regulatory- and degradative-type proteases, impacting a variety of, physiological processes including regulation of blood pressure, neuronal, health, and the inflammatory response. While the function of the majority, of the kallikreins remains to be elucidated, two members are useful, biomarkers for prostate cancer and several others are potentially useful, biomarkers for breast cancer, Alzheimer's, and Parkinson's disease. Human, tissue kallikrein (human K1) is the best functionally characterized member, of this family, and is known to play an important role in blood pressure, regulation. As part of this function, human K1 exhibits unique, dual-substrate specificity in hydrolyzing low molecular weight kininogen, between both Arg-Ser and Met-Lys sequences. We report the X-ray crystal, structure of mature, active recombinant human apo K1 at 1.70 A resolution., The active site exhibits structural features intermediate between that of, apo and pro forms of known kallikrein structures. The S2 to S2' pockets, demonstrate a variety of conformational changes in comparison to the, porcine homolog of K1 in complex with peptide inhibitors, including the, displacement of an extensive solvent network. These results indicate that, the binding of a peptide substrate contributes to a structural, rearrangement of the active-site Ser 195 resulting in a catalytically, competent juxtaposition with the active-site His 57. The solvent networks, within the S1 and S1' pockets suggest how the Arg-Ser and Met-Lys dual, substrate specificity of human K1 is accommodated.
Disease
Known disease associated with this structure: Kallikrein, decreased urinary activity of OMIM:[147910]
About this Structure
1SPJ is a Single protein structure of sequence from Homo sapiens with NAG, CA and ACY as ligands. Active as Tissue kallikrein, with EC number 3.4.21.35 Full crystallographic information is available from OCA.
Reference
1.70 A X-ray structure of human apo kallikrein 1: structural changes upon peptide inhibitor/substrate binding., Laxmikanthan G, Blaber SI, Bernett MJ, Scarisbrick IA, Juliano MA, Blaber M, Proteins. 2005 Mar 1;58(4):802-14. PMID:15651049
Page seeded by OCA on Mon Nov 12 19:16:41 2007
