4pqg

Structural highlights

Function

GTFA_STRPN Required for the polymorphic O-glycosylation of serine-rich repeat protein PsrP. Catalyzes the first step in glycosylation by transferring N-acetylglucosamine from UDP-GlcNAc to serine residues in PsrP (PubMed:24936067, PubMed:28246170). Part of the accessory SecA2/SecY2 system specifically required to export serine-rich repeat cell wall proteins encoded upstream in the same operon (Probable). The GtfA-GtfB complex adds GlcNAc from UDP-GlcNAc to PsrP (experimentally characterized with truncated PsrP-SSR1 constructs); this subunit alone has weak N-acetylglucosaminyl transferase activity that is 10-fold stimulated by GtfB. The complex requires at least a 25 residue-long peptide for activity; the in vitro assay has only been seen to glycosylate Ser residues (PubMed:24936067). The alpha linkage was shown in L.reuteri.[UniProtKB:A0A0S4NM89]^{[1] [2] [3]}

See Also

• Glycosyltransferase 3D structures
• O-GlcNAc transferase 3D structures

References

1. ↑ Shi WW, Jiang YL, Zhu F, Yang YH, Shao QY, Yang HB, Ren YM, Wu H, Chen Y, Zhou CZ. Structure of a Novel O-Linked N-Acetyl-d-glucosamine (O-GlcNAc) Transferase, GtfA, Reveals Insights into the Glycosylation of Pneumococcal Serine-rich Repeat Adhesins. J Biol Chem. 2014 Jul 25;289(30):20898-20907. Epub 2014 Jun 16. PMID:24936067 doi:http://dx.doi.org/10.1074/jbc.M114.581934
2. ↑ Jiang YL, Jin H, Yang HB, Zhao RL, Wang S, Chen Y, Zhou CZ. Defining the enzymatic pathway for polymorphic O-glycosylation of the pneumococcal serine-rich repeat protein PsrP. J Biol Chem. 2017 Apr 14;292(15):6213-6224. doi: 10.1074/jbc.M116.770446. Epub, 2017 Feb 28. PMID:28246170 doi:http://dx.doi.org/10.1074/jbc.M116.770446
3. ↑ Obert C, Sublett J, Kaushal D, Hinojosa E, Barton T, Tuomanen EI, Orihuela CJ. Identification of a Candidate Streptococcus pneumoniae core genome and regions of diversity correlated with invasive pneumococcal disease. Infect Immun. 2006 Aug;74(8):4766-77. PMID:16861665 doi:10.1128/IAI.00316-06