8pol

Structural highlights

Function

SUB1_PLAF7 Serine protease which plays an essential role in merozoite invasion of and egress from host erythrocytes by processing and activating various merozoite surface and parasitophorous vacuole proteins (PubMed:18083098, PubMed:19214190, PubMed:21220481, PubMed:29459732). Mediates the proteolytic maturation of serine proteases SERA4, SERA5 and SERA6 just prior to merozoite egress (PubMed:18083098, PubMed:19214190, PubMed:29459732). Prior to merozoite egress, cleaves merozoite surface proteins MSP1, MSP6 and MSP7, which form the MSP1/6/7 complex, and thereby may prime the parasite cell surface for invasion of fresh erythrocytes (PubMed:19214190, PubMed:29459732). Prior to merozoite egress, cleaves MSRP2 converting it to MSRP2 p25 form, and RAP1 converting it to RAP1 p67 form (PubMed:21220481).^{[1] [2] [3] [4]}

References

1. ↑ Yeoh S, O'Donnell RA, Koussis K, Dluzewski AR, Ansell KH, Osborne SA, Hackett F, Withers-Martinez C, Mitchell GH, Bannister LH, Bryans JS, Kettleborough CA, Blackman MJ. Subcellular discharge of a serine protease mediates release of invasive malaria parasites from host erythrocytes. Cell. 2007 Dec 14;131(6):1072-83. PMID:18083098 doi:10.1016/j.cell.2007.10.049
2. ↑ Koussis K, Withers-Martinez C, Yeoh S, Child M, Hackett F, Knuepfer E, Juliano L, Woehlbier U, Bujard H, Blackman MJ. A multifunctional serine protease primes the malaria parasite for red blood cell invasion. EMBO J. 2009 Mar 18;28(6):725-35. PMID:19214190 doi:10.1038/emboj.2009.22
3. ↑ Silmon de Monerri NC, Flynn HR, Campos MG, Hackett F, Koussis K, Withers-Martinez C, Skehel JM, Blackman MJ. Global identification of multiple substrates for Plasmodium falciparum SUB1, an essential malarial processing protease. Infect Immun. 2011 Mar;79(3):1086-97. PMID:21220481 doi:10.1128/IAI.00902-10
4. ↑ Thomas JA, Tan MSY, Bisson C, Borg A, Umrekar TR, Hackett F, Hale VL, Vizcay-Barrena G, Fleck RA, Snijders AP, Saibil HR, Blackman MJ. A protease cascade regulates release of the human malaria parasite Plasmodium falciparum from host red blood cells. Nat Microbiol. 2018 Apr;3(4):447-455. PMID:29459732 doi:10.1038/s41564-018-0111-0