Structural highlights
Function
GCI_AGRFC Catalyzes the ring opening of D-galactaro-1,4-lactone to yield 5-keto-4-deoxy-D-glucarate (KDG) via a beta-elimination reaction. This is a step in the oxidative degradation pathway of D-galacturonate, which allows A.tumefaciens to utilize D-galacturonate as a sole carbon source. To a lesser extent, can also use D-glucaro-1,4-lactone as substrate to produce KDG, but cannot use D-galactaro-1,5-lactone, D-glucaro-6,3-lactone and linear D-glucarate.[1] [2]
References
- ↑ Andberg M, Maaheimo H, Boer H, Penttila M, Koivula A, Richard P. Characterization of a novel Agrobacterium tumefaciens galactarolactone cycloisomerase enzyme for direct conversion of D-galactarolactone to 3-deoxy-2-keto-L-threo-hexarate. J Biol Chem. 2012 May 18;287(21):17662-71. doi: 10.1074/jbc.M111.335240. Epub, 2012 Apr 5. PMID:22493433 doi:http://dx.doi.org/10.1074/jbc.M111.335240
- ↑ Bouvier JT, Groninger-Poe FP, Vetting M, Almo SC, Gerlt JA. Galactaro delta-lactone isomerase: lactone isomerization by a member of the amidohydrolase superfamily. Biochemistry. 2014 Feb 4;53(4):614-6. doi: 10.1021/bi5000492. Epub 2014 Jan 24. PMID:24450804 doi:http://dx.doi.org/10.1021/bi5000492
