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Merritt's Take on DPPIV

spinqualitylabelsall models Dipeptidyl Peptidase IV (6B1E) Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image This is a default text for your page Merritt Jugo/Sandbox 1. Click above on edit this page to modify. Be careful with the < and > signs. You may include any references to papers as in: the use of JSmol in Proteopedia [1] or to the article describing Jmol [2] to the rescue. Introduction The RNA lariat debranching enzyme, Dbr1, is a metallophosphoesterase that cleaves 2'-5' phosphodiester bonds within intronic lariats. [3] Previous reports have indicated that Dbr1 enzymatic activity is supported by diverse metal ions including Ni2+ , Mn2+ , Mg2+ , Fe2+ , and Zn2+ . [4]

References

1. ↑ Hanson, R. M., Prilusky, J., Renjian, Z., Nakane, T. and Sussman, J. L. (2013), JSmol and the Next-Generation Web-Based Representation of 3D Molecular Structure as Applied to Proteopedia. Isr. J. Chem., 53:207-216. doi:http://dx.doi.org/10.1002/ijch.201300024
2. ↑ Herraez A. Biomolecules in the computer: Jmol to the rescue. Biochem Mol Biol Educ. 2006 Jul;34(4):255-61. doi: 10.1002/bmb.2006.494034042644. PMID:21638687 doi:10.1002/bmb.2006.494034042644
3. ↑ Ransey E, Paredes E, Dey SK, Das SR, Heroux A, Macbeth MR. Crystal structure of the Entamoeba histolytica RNA lariat debranching enzyme EhDbr1 reveals a catalytic Zn(2+) /Mn(2+) heterobinucleation. FEBS Lett. 2017 Jul;591(13):2003-2010. doi: 10.1002/1873-3468.12677. Epub 2017, Jun 14. PMID:28504306 doi:http://dx.doi.org/10.1002/1873-3468.12677
4. ↑ ICP-AES corroborate this finding, and in vitro debranching assays with fluorescently labeled branched substrates confirm activity. <ref> ==Function== ===Catalytic Triad=== The [https://en.wikipedia.org/wiki/Catalytic_triad#:~:text=A%20catalytic%20triad%20is%20a,lipases%20and%20β%2Dlactamases). Catalytic Triad] of DPP4 is Ser630, His740, Asp708. ===Mechanism=== [[Image:DPPIV_Mech.png|400 px|right|thumb|Figure 1. The coolest image of this protein EVAH!!!]] ==Disease== ==Relevance== ==Structural highlights== <scene name='10/1037493/Beta_propeller_in_white/7'>Beta Propeller</scene> This is a sample scene created with SAT to <scene name="/12/3456/Sample/1">color</scene> by Group, and another to make <scene name="/12/3456/Sample/2">a transparent representation</scene> of the protein. You can make your own scenes on SAT starting from scratch or loading and editing one of these sample scenes. </li></ol></ref>

Student Contributors

• Merritt Jugo