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1am5

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Revision as of 16:27, 29 October 2007 by OCA (Talk | contribs)
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1am5, resolution 2.16Å

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THE CRYSTAL STRUCTURE AND PROPOSED AMINO ACID SEQUENCE OF A PEPSIN FROM ATLANTIC COD (GADUS MORHUA)

Overview

The crystal structure of a pepsin from the gastric mucosa of Atlantic cod, has been determined to 2.16 A resolution. Data were collected on, orthorhombic crystals with cell dimensions a = 35.98, b = 75.40 and c =, 108.10 A, on a FAST area-detector system. The phase problem was solved by, the molecular-replacement method using porcine pepsin (PDB entry 5PEP) as, a search model. The structure has been refined to a crystallographic R, factor of 20.8% using all reflections between 8.0 and 2.16 A, without, prior knowledge of the primary sequence. The resulting crystal structure, is very similar to the porcine enzyme, consisting of two domains with, predominantly beta-sheet structure in the same sequential positions as the, enzyme from pig. In the course of the model building, 122 residues ... [(full description)]

About this Structure

1AM5 is a [Single protein] structure of sequence from [Gadus morhua]. Active as [[1]], with EC number [3.4.23.1]. Full crystallographic information is available from [OCA].

Reference

Structure and proposed amino-acid sequence of a pepsin from atlantic cod (Gadus morhua)., Karlsen S, Hough E, Olsen RL, Acta Crystallogr D Biol Crystallogr. 1998 Jan 1;54(Pt 1):32-46. PMID:9761815

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