1p3w

Structural highlights

Function

ISCS_ECOLI Master enzyme that delivers sulfur to a number of partners involved in Fe-S cluster assembly, tRNA modification or cofactor biosynthesis. Catalyzes the removal of elemental sulfur from cysteine to produce alanine. Functions as a sulfur delivery protein for Fe-S cluster synthesis onto IscU, an Fe-S scaffold assembly protein, as well as other S acceptor proteins. Preferentially binds to disordered IscU on which the Fe-S is assembled, IscU converts to the structured state and then dissociates from IscS to transfer the Fe-S to an acceptor protein. Also functions as a selenium delivery protein in the pathway for the biosynthesis of selenophosphate. Transfers sulfur onto 'Cys-456' of ThiI and onto 'Cys-19' of TusA in transpersulfidation reactions.^{[1] [2] [3] [4] [5]}

Evolutionary Conservation

Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf.

See Also

• Cysteine desulfurase 3D structures

References

1. ↑ Flint DH. Escherichia coli contains a protein that is homologous in function and N-terminal sequence to the protein encoded by the nifS gene of Azotobacter vinelandii and that can participate in the synthesis of the Fe-S cluster of dihydroxy-acid dehydratase. J Biol Chem. 1996 Jul 5;271(27):16068-74. PMID:8663056
2. ↑ Lacourciere GM, Mihara H, Kurihara T, Esaki N, Stadtman TC. Escherichia coli NifS-like proteins provide selenium in the pathway for the biosynthesis of selenophosphate. J Biol Chem. 2000 Aug 4;275(31):23769-73. PMID:10829016 doi:10.1074/jbc.M000926200
3. ↑ Urbina HD, Silberg JJ, Hoff KG, Vickery LE. Transfer of sulfur from IscS to IscU during Fe/S cluster assembly. J Biol Chem. 2001 Nov 30;276(48):44521-6. Epub 2001 Sep 27. PMID:11577100 doi:http://dx.doi.org/10.1074/jbc.M106907200
4. ↑ Ikeuchi Y, Shigi N, Kato J, Nishimura A, Suzuki T. Mechanistic insights into sulfur relay by multiple sulfur mediators involved in thiouridine biosynthesis at tRNA wobble positions. Mol Cell. 2006 Jan 6;21(1):97-108. PMID:16387657 doi:http://dx.doi.org/S1097-2765(05)01761-2
5. ↑ Kim JH, Tonelli M, Markley JL. Disordered form of the scaffold protein IscU is the substrate for iron-sulfur cluster assembly on cysteine desulfurase. Proc Natl Acad Sci U S A. 2012 Jan 10;109(2):454-9. doi: 10.1073/pnas.1114372109., Epub 2011 Dec 27. PMID:22203963 doi:http://dx.doi.org/10.1073/pnas.1114372109