Structural highlights
Function
ACT1_PLAF7 Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Actin assembles into short polymer microfilaments, these are thought to contribute to parasite gliding motility.
Publication Abstract from PubMed
Cadmium ions can be effectively used to promote crystal growth and for experimental phasing. Here, the use of cadmium ions as a suitable anomalous scatterer at the standard wavelength of 1 A is demonstrated. The structures of three different proteins were determined using cadmium single-wavelength anomalous dispersion (SAD) phasing. Owing to the strong anomalous signal, the structure of lysozyme could be automatically phased and built using a very low anomalous multiplicity (1.1) and low-completeness (77%) data set. Additionally, it is shown that cadmium ions can easily substitute divalent ions in ATP-divalent cation complexes. This property could be generally applied for phasing experiments of a wide range of nucleotide-binding proteins. Improvements in crystal growth and quality, good anomalous signal at standard wavelengths (i.e. no need to change photon energy) and rapid phasing and refinement using a single data set are benefits that should allow cadmium ions to be widely used for experimental phasing.
Rapid cadmium SAD phasing at the standard wavelength (1 A).,Panneerselvam S, Kumpula EP, Kursula I, Burkhardt A, Meents A Acta Crystallogr D Struct Biol. 2017 Jul 1;73(Pt 7):581-590. doi:, 10.1107/S2059798317006970. Epub 2017 Jun 30. PMID:28695858[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
See Also
References
- ↑ Panneerselvam S, Kumpula EP, Kursula I, Burkhardt A, Meents A. Rapid cadmium SAD phasing at the standard wavelength (1 A). Acta Crystallogr D Struct Biol. 2017 Jul 1;73(Pt 7):581-590. doi:, 10.1107/S2059798317006970. Epub 2017 Jun 30. PMID:28695858 doi:http://dx.doi.org/10.1107/S2059798317006970
