Structural highlights
Function
CPRC1_CANPA NADPH-dependent conjugated polyketone reductase with broad substrate specificity and strict stereospecificity. Reduces ketopantoyl lactone (2-dehydropantolactone) and isatin. Does not act on menadione, p-nitrobenzaldehyde and pyridine-3-aldehyde.[1] [2]
Publication Abstract from PubMed
Conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 is a member of the aldo-keto reductase (AKR) superfamily and reduces ketopantoyl lactone to D-pantoyl lactone in a NADPH-dependent and stereospecific manner. We determined the crystal structure of CPR-C1.NADPH complex at 2.20 A resolution. CPR-C1 adopted a triose-phosphate isomerase (TIM) barrel fold at the core of the structure in which Thr25 and Lys26 of the GXGTX motif bind uniquely to the adenosine 2'-phosphate group of NADPH. This finding provides a novel structural basis for NADPH binding of the AKR superfamily. (c) Proteins 2013;. (c) 2013 Wiley Periodicals, Inc.
Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH.,Qin HM, Yamamura A, Miyakawa T, Kataoka M, Maruoka S, Ohtsuka J, Nagata K, Shimizu S, Tanokura M Proteins. 2013 Jul 15. doi: 10.1002/prot.24363. PMID:23852710[3]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Hidalgo AR, Akond MA, Kita K, Kataoka M, Shimizu S. Isolation and primary structural analysis of two conjugated polyketone reductases from Candida parapsilosis. Biosci Biotechnol Biochem. 2001 Dec;65(12):2785-8. doi: 10.1271/bbb.65.2785. PMID:11826979 doi:http://dx.doi.org/10.1271/bbb.65.2785
- ↑ Kataoka M, Delacruz-Hidalgo AR, Akond MA, Sakuradani E, Kita K, Shimizu S. Gene cloning and overexpression of two conjugated polyketone reductases, novel aldo-keto reductase family enzymes, of Candida parapsilosis. Appl Microbiol Biotechnol. 2004 Apr;64(3):359-66. doi: 10.1007/s00253-003-1484-3., Epub 2003 Oct 31. PMID:14593510 doi:http://dx.doi.org/10.1007/s00253-003-1484-3
- ↑ Qin HM, Yamamura A, Miyakawa T, Kataoka M, Maruoka S, Ohtsuka J, Nagata K, Shimizu S, Tanokura M. Crystal structure of conjugated polyketone reductase (CPR-C1) from Candida parapsilosis IFO 0708 complexed with NADPH. Proteins. 2013 Jul 15. doi: 10.1002/prot.24363. PMID:23852710 doi:10.1002/prot.24363
