Structural highlights
Function
ACER_ACIBT Regulates the expression of the AceI transporter (PubMed:29481596). Binds DNA and chlorhexidine (PubMed:29481596, PubMed:32636271). Binds to regulatory sites within the intergenic region between the aceI and aceR genes, and affects the interaction between RNA polymerase (RNAP) and promoter DNA both in the presence and in the absence of chlorhexidine (PubMed:29481596, PubMed:32636271). In the absence of chlorhexidine, prevents transcription of the aceI gene by disrupting interactions between the promoter DNA and RNAP (PubMed:32636271). In the presence of chlorhexidine, activates expression of aceI (PubMed:29481596, PubMed:32636271). When AceR interacts with chlorhexidine, it undergoes a conformational change and the tetrameric form either releases the DNA or shifts the position of the DNA-binding region to allow RNAP to bind onto the promoter DNA to proceed with aceI transcription (PubMed:32636271).[1] [2]
References
- ↑ Liu Q, Hassan KA, Ashwood HE, Gamage HKAH, Li L, Mabbutt BC, Paulsen IT. Regulation of the aceI multidrug efflux pump gene in Acinetobacter baumannii. J Antimicrob Chemother. 2018 Jun 1;73(6):1492-1500. PMID:29481596 doi:10.1093/jac/dky034
- ↑ Bolla JR, Howes AC, Fiorentino F, Robinson CV. Assembly and regulation of the chlorhexidine-specific efflux pump AceI. Proc Natl Acad Sci U S A. 2020 Jul 21;117(29):17011-17018. PMID:32636271 doi:10.1073/pnas.2003271117
