Structural highlights
Function
B0I564_CHICK
Publication Abstract from PubMed
DNA interstrand cross-links are tumor-inducing lesions that block DNA replication and transcription. When cross-links are detected at stalled replication forks, ATR kinase phosphorylates FANCI, which stimulates monoubiquitination of the FANCD2-FANCI clamp by the Fanconi anemia core complex. Monoubiquitinated FANCD2-FANCI is locked onto DNA and recruits nucleases that mediate DNA repair. However, it remains unclear how phosphorylation activates this pathway. Here, we report structures of FANCD2-FANCI complexes containing phosphomimetic FANCI. We observe that, unlike wild-type FANCD2-FANCI, the phosphomimetic complex closes around DNA, independent of the Fanconi anemia core complex. The phosphomimetic mutations do not substantially alter DNA binding but instead destabilize the open state of FANCD2-FANCI and alter its conformational dynamics. Overall, our results demonstrate that phosphorylation primes the FANCD2-FANCI clamp for ubiquitination, showing how multiple posttranslational modifications are coordinated to control DNA repair.
The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination.,Sijacki T, Alcon P, Chen ZA, McLaughlin SH, Shakeel S, Rappsilber J, Passmore LA Nat Struct Mol Biol. 2022 Sep;29(9):881-890. doi: 10.1038/s41594-022-00820-9. , Epub 2022 Sep 1. PMID:36050501[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Sijacki T, Alcón P, Chen ZA, McLaughlin SH, Shakeel S, Rappsilber J, Passmore LA. The DNA-damage kinase ATR activates the FANCD2-FANCI clamp by priming it for ubiquitination. Nat Struct Mol Biol. 2022 Sep;29(9):881-890. PMID:36050501 doi:10.1038/s41594-022-00820-9
