Structural highlights
Function
A0A1S4NYF2_BRALA A0A254U4M1_ASPNG A0A100IBH9_ASPNG
Publication Abstract from PubMed
Neurobiologists widely use green genetically encoded calcium indicators (GECIs) for visualization of neuronal activity. Among them, ratiometric GECIs allow imaging of both active and non-active neuronal populations. However, they are not popular, since their properties are inferior to intensiometric GCaMP series of GECIs. The most characterized and developed ratiometric green GECI is FGCaMP7. However, the dynamic range and sensitivity of its large Stock's shift green (LSS-Green) form is significantly lower than its Green form and its molecular design is not optimal. To address these drawbacks, we engineered a ratiometric green calcium indicator, called FNCaMP, which is based on bright mNeonGreen protein and calmodulin from A. niger and has optimal NTnC-like design. We compared the properties of the FNCaMP and FGCaMP7 indicators in vitro, in mammalian cells, and in neuronal cultures. Finally, we obtained and analyzed X-ray structure of the FNCaMP indicator.
FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein.,Subach OM, Varfolomeeva L, Vlaskina AV, Agapova YK, Nikolaeva AY, Piatkevich KD, Patrushev MV, Boyko KM, Subach FV Biochem Biophys Res Commun. 2023 Jul 12;665:169-177. doi: , 10.1016/j.bbrc.2023.04.108. Epub 2023 Apr 28. PMID:37163937[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Subach OM, Varfolomeeva L, Vlaskina AV, Agapova YK, Nikolaeva AY, Piatkevich KD, Patrushev MV, Boyko KM, Subach FV. FNCaMP, ratiometric green calcium indicator based on mNeonGreen protein. Biochem Biophys Res Commun. 2023 Apr 28;665:169-177. PMID:37163937 doi:10.1016/j.bbrc.2023.04.108
