1t3j
From Proteopedia
Mitofusin domain HR2 V686M/I708M mutant
Overview
Vesicle fusion involves vesicle tethering, docking, and membrane merger. We show that mitofusin, an integral mitochondrial membrane protein, is required on adjacent mitochondria to mediate fusion, which indicates that mitofusin complexes act in trans (that is, between adjacent mitochondria). A heptad repeat region (HR2) mediates mitofusin oligomerization by assembling a dimeric, antiparallel coiled coil. The transmembrane segments are located at opposite ends of the 95 angstrom coiled coil and provide a mechanism for organelle tethering. Consistent with this proposal, truncated mitofusin, in an HR2-dependent manner, causes mitochondria to become apposed with a uniform gap. Our results suggest that HR2 functions as a mitochondrial tether before fusion.
About this Structure
1T3J is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Structural basis of mitochondrial tethering by mitofusin complexes., Koshiba T, Detmer SA, Kaiser JT, Chen H, McCaffery JM, Chan DC, Science. 2004 Aug 6;305(5685):858-62. PMID:15297672 Page seeded by OCA on Sat May 3 09:28:36 2008
