6h79

Publication Abstract from PubMed

Serial synchrotron crystallography allows low X-ray dose, room-temperature crystal structures of proteins to be determined from a population of microcrystals. Protein production and crystallization is a non-trivial procedure and it is essential to have X-ray-compatible sample environments that keep sample consumption low and the crystals in their native environment. This article presents a fast and optimized manufacturing route to metal-polyimide microfluidic flow-focusing devices which allow for the collection of X-ray diffraction data in flow. The flow-focusing conditions allow for sample consumption to be significantly decreased, while also opening up the possibility of more complex experiments such as rapid mixing for time-resolved serial crystallography. This high-repetition-rate experiment allows for full datasets to be obtained quickly ( approximately 1 h) from crystal slurries in liquid flow. The X-ray compatible microfluidic chips are easily manufacturable, reliable and durable and require sample-flow rates on the order of only 30 microl h(-1).

A microfluidic flow-focusing device for low sample consumption serial synchrotron crystallography experiments in liquid flow.,Monteiro DCF, Vakili M, Harich J, Sztucki M, Meier SM, Horrell S, Josts I, Trebbin M J Synchrotron Radiat. 2019 Mar 1;26(Pt 2):406-412. doi:, 10.1107/S1600577519000304. Epub 2019 Feb 13. PMID:30855249^[2]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.