7t9q
From Proteopedia
Crystal structure of Crocodile defensin CpoBD13
Structural highlights
FunctionDFB13_CROPO Exhibits antimicrobial activity against fungi (PubMed:36859344). Antimicrobial activity in a pH-dependent manner against the yeast C.albicans; activity is salt tolerant and retains antifungal activity in NaCl concentrations of 100mM (PubMed:36859344). Permeabilizes C.albicans cell membranes via targeting plasma membrane phospholipid phosphatidic acid (PubMed:36859344).[1] Publication Abstract from PubMedCrocodilians are an order of ancient reptiles that thrive in pathogen-rich environments. The ability to inhabit these harsh environments is indicative of a resilient innate immune system. Defensins, a family of cysteine-rich cationic host defence peptides, are a major component of the innate immune systems of all plant and animal species, however crocodilian defensins are poorly characterised. We now show that the saltwater crocodile defensin CpoBD13 harbors potent antifungal activity that is mediated by a pH-dependent membrane-targeting action. CpoBD13 binds the phospholipid phosphatidic acid (PA) to form a large helical oligomeric complex, with specific histidine residues mediating PA binding. The utilisation of histidine residues for PA engagement allows CpoBD13 to exhibit differential activity at a range of environmental pH values, where CpoBD13 is optimally active in an acidic environment. Crocodile defensin (CpoBD13) antifungal activity via pH-dependent phospholipid targeting and membrane disruption.,Williams SA, Lay FT, Bindra GK, Banjara S, Poon IKH, Phan TK, Kvansakul M, Hulett MD Nat Commun. 2023 Mar 1;14(1):1170. doi: 10.1038/s41467-023-36280-y. PMID:36859344[2] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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