2b9l
From Proteopedia
Crystal structure of prophenoloxidase activating factor-II from the beetle Holotrichia diomphalia
Structural highlights
FunctionPPAF2_HOLDI Binds and activates processed prophenoloxidases PPO1 and PPO2 and thus is involved in the activation of the prophenoloxidase cascade probably following the recognition of pathogen-derived products.[1] [2] [3] [4] [5] Evolutionary Conservation Check, as determined by ConSurfDB. You may read the explanation of the method and the full data available from ConSurf. Publication Abstract from PubMedClip-domain serine proteases (SPs) are the essential components of extracellular signaling cascades in various biological processes, especially in embryonic development and the innate immune responses of invertebrates. They consist of a chymotrypsin-like SP domain and one or two clip domains at the N-terminus. Prophenoloxidase-activating factor (PPAF)-II, which belongs to the noncatalytic clip-domain SP family, is indispensable for the generation of the active phenoloxidase leading to melanization, a major defense mechanism of insects. Here, the crystal structure of PPAF-II reveals that the clip domain adopts a novel fold containing a central cleft, which is distinct from the structures of defensins with a similar arrangement of cysteine residues. Ensuing studies demonstrated that PPAF-II forms a homo-oligomer upon cleavage by the upstream protease and that the clip domain of PPAF-II functions as a module for binding phenoloxidase through the central cleft, while the clip domain of a catalytically active easter-type SP plays an essential role in the rapid activation of its protease domain. Crystal structure of a clip-domain serine protease and functional roles of the clip domains.,Piao S, Song YL, Kim JH, Park SY, Park JW, Lee BL, Oh BH, Ha NC EMBO J. 2005 Dec 21;24(24):4404-14. Epub 2005 Dec 15. PMID:16362048[6] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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Categories: Holotrichia diomphalia | Large Structures | Ha N-C | Lee BL | Oh B-H | Park SY | Piao S | Song Y-L
