Structural highlights
Publication Abstract from PubMed
We introduce Cryogenic Optical Localization in 3D (COLD), a method to localize multiple fluorescent sites within a single small protein with Angstrom resolution. We demonstrate COLD by determining the conformational state of the cytosolic Per-ARNT-Sim domain from the histidine kinase CitA of Geobacillus thermodenitrificans and resolving the four biotin sites of streptavidin. COLD provides quantitative 3D information about small- to medium-sized biomolecules on the Angstrom scale and complements other techniques in structural biology.
Cryogenic optical localization provides 3D protein structure data with Angstrom resolution.,Weisenburger S, Boening D, Schomburg B, Giller K, Becker S, Griesinger C, Sandoghdar V Nat Methods. 2017 Feb;14(2):141-144. doi: 10.1038/nmeth.4141. Epub 2017 Jan 9. PMID:28068317[1]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Weisenburger S, Boening D, Schomburg B, Giller K, Becker S, Griesinger C, Sandoghdar V. Cryogenic optical localization provides 3D protein structure data with Angstrom resolution. Nat Methods. 2017 Feb;14(2):141-144. doi: 10.1038/nmeth.4141. Epub 2017 Jan 9. PMID:28068317 doi:http://dx.doi.org/10.1038/nmeth.4141
