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Publication Abstract from PubMed

Chemical modifications on histones and DNA/RNA constitute a fundamental mechanism for epigenetic regulation. These modifications often function as docking marks to recruit or stabilize cognate "reader" proteins. So far, a platform for quantitative and high-throughput profiling of the epigenetic interactome is urgently needed but still lacking. Here, we report a 3D-carbene chip-based surface plasmon resonance imaging (SPRi) technology for this purpose. The 3D-carbene chip is suitable for immobilizing versatile biomolecules (e.g., peptides, antibody, DNA/RNA) and features low nonspecific binding, random yet function-retaining immobilization, and robustness for reuses. We systematically profiled binding kinetics of 1,000 histone "reader-mark" pairs on a single 3D-carbene chip and validated two recognition events by calorimetric and structural studies. Notably, a discovery on H3K4me3 recognition by the DNA mismatch repair protein MSH6 in Capsella rubella suggests a mechanism of H3K4me3-mediated DNA damage repair in plant.

Kinetic and high-throughput profiling of epigenetic interactions by 3D-carbene chip-based surface plasmon resonance imaging technology.,Zhao S, Yang M, Zhou W, Zhang B, Cheng Z, Huang J, Zhang M, Wang Z, Wang R, Chen Z, Zhu J, Li H Proc Natl Acad Sci U S A. 2017 Aug 29;114(35):E7245-E7254. doi:, 10.1073/pnas.1704155114. Epub 2017 Aug 14. PMID:28808021^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.