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Publication Abstract from PubMed

ParE(SO)-CopA(SO) is a new type II toxin-antitoxin (TA) system in prophage CP4So that plays an essential role in circular CP4So maintenance after the excision in Shewanella oneidensis. The toxin ParE(SO) severely inhibits cell growth, while CopA(SO) functions as an antitoxin to neutralize ParE(SO) toxicity through direct interactions. However, the molecular mechanism of the neutralization and autoregulation of the TA operon transcription remains elusive. In this study, we determined the crystal structure of a ParE(SO)-CopA(SO) complex that adopted an open V-shaped heterotetramer with the organization of ParE(SO)-(CopA(SO))(2)-ParE(SO). The structure showed that upon ParE(SO) binding, the intrinsically disordered C-terminal domain of CopA(SO) was induced to fold into a partially ordered conformation that bound into a positively charged and hydrophobic groove of ParE(SO). Thermodynamics analysis showed the DNA-binding affinity of CopA(SO) was remarkably higher than that of the purified TA complex, accompanied by the enthalpy change reversion from an exothermic reaction to an endothermic reaction. These results suggested ParE(SO) acts as a de-repressor of the TA operon transcription at the toxin:antitoxin level of 1:1. Site-directed mutagenesis of ParE(SO) identified His91 as the essential residue for its toxicity by cell toxicity assays. Our structure-function studies therefore elucidated the transcriptional regulation mechanism of the ParE(SO)-CopA(SO) pair, and may help to understand the regulation of CP4So maintenance in S. oneidensis.

Insights into the Neutralization and DNA Binding of Toxin-Antitoxin System ParE(SO)-CopA(SO) by Structure-Function Studies.,Zhou J, Du XJ, Liu Y, Gao ZQ, Geng Z, Dong YH, Zhang H Microorganisms. 2021 Dec 3;9(12):2506. doi: 10.3390/microorganisms9122506. PMID:34946107^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.