8xsu
From Proteopedia
Crystal Structure of Lymnaea stagnalis Acetylcholine-Binding Protein Q55R Mutant Complexed with Dinotefuran
Structural highlights
FunctionACHP_LYMST Binds to acetylcholine. Modulates neuronal synaptic transmission. Publication Abstract from PubMedWith the spread of resistance to long-established insecticides targeting Anopheles malaria vectors, understanding the actions of compounds newly identified for vector control is essential. With new commercial vector-control products containing neonicotinoids under development, we investigate the actions of 6 neonicotinoids (imidacloprid, thiacloprid, clothianidin, dinotefuran, nitenpyram and acetamiprid) on 13 Anopheles gambiae nicotinic acetylcholine receptor (nAChR) subtypes produced by expression of combinations of the Agalpha1, Agalpha2, Agalpha3, Agalpha8 and Agbeta1 subunits in Xenopus laevis oocytes, the Drosophila melanogaster orthologues of which we have previously shown to be important in neonicotinoid actions. The presence of the Agalpha2 subunit reduces neonicotinoid affinity for the mosquito nAChRs, whereas the Agalpha3 subunit increases it. Crystal structures of the acetylcholine binding protein (AChBP), an established surrogate for the ligand-binding domain, with dinotefuran bound, shows a unique target site interaction through hydrogen bond formation and CH-N interaction at the tetrahydrofuran ring. This is of interest as dinotefuran is also under trial as the toxic element in baited traps. Multiple regression analyses show a correlation between the efficacy of neonicotinoids for the Agalpha1/Agalpha2/Agalpha8/Agbeta1 nAChR, their hydrophobicity and their rate of knockdown of adult female An. gambiae, providing new insights into neonicotinoid features important for malaria vector control. Unravelling nicotinic receptor and ligand features underlying neonicotinoid knockdown actions on the malaria vector mosquito Anopheles gambiae.,Ito R, Kamiya M, Takayama K, Mori S, Matsumoto R, Takebayashi M, Ojima H, Fujimura S, Yamamoto H, Ohno M, Ihara M, Okajima T, Yamashita A, Colman F, Lycett GJ, Sattelle DB, Matsuda K Open Biol. 2024 Jul;14(7):240057. doi: 10.1098/rsob.240057. Epub 2024 Jul 24. PMID:39043224[1] From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine. References
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