9fms

Structural highlights

Function

XRN2_YEAST Possesses 5'->3' exoribonuclease activity. Required for the processing of nuclear mRNA, rRNA and small nucleolar RNA (snoRNA) precursors. May promote termination of transcription by RNA polymerase II via the recruitment of 3'-end processing factors to the poly(A) site and by the degradation of nascent RNA downstream of the poly(A) site.^{[1] [2] [3] [4] [5] [6] [7] [8] [9] [10] [11] [12] [13] [14] [15] [16]}

References

1. ↑ Xue Y, Bai X, Lee I, Kallstrom G, Ho J, Brown J, Stevens A, Johnson AW. Saccharomyces cerevisiae RAI1 (YGL246c) is homologous to human DOM3Z and encodes a protein that binds the nuclear exoribonuclease Rat1p. Mol Cell Biol. 2000 Jun;20(11):4006-15. PMID:10805743 doi:10.1128/MCB.20.11.4006-4015.2000
2. ↑ Bousquet-Antonelli C, Presutti C, Tollervey D. Identification of a regulated pathway for nuclear pre-mRNA turnover. Cell. 2000 Sep 15;102(6):765-75. PMID:11030620 doi:10.1016/s0092-8674(00)00065-9
3. ↑ Geerlings TH, Vos JC, Raue HA. The final step in the formation of 25S rRNA in Saccharomyces cerevisiae is performed by 5'-->3' exonucleases. RNA. 2000 Dec;6(12):1698-703. PMID:11142370
4. ↑ He F, Jacobson A. Upf1p, Nmd2p, and Upf3p regulate the decapping and exonucleolytic degradation of both nonsense-containing mRNAs and wild-type mRNAs. Mol Cell Biol. 2001 Mar;21(5):1515-30. PMID:11238889 doi:http://dx.doi.org/10.1128/MCB.21.5.1515-1530.2001
5. ↑ Danin-Kreiselman M, Lee CY, Chanfreau G. RNAse III-mediated degradation of unspliced pre-mRNAs and lariat introns. Mol Cell. 2003 May;11(5):1279-89. PMID:12769851 doi:10.1016/s1097-2765(03)00137-0
6. ↑ Lee CY, Lee A, Chanfreau G. The roles of endonucleolytic cleavage and exonucleolytic digestion in the 5'-end processing of S. cerevisiae box C/D snoRNAs. RNA. 2003 Nov;9(11):1362-70. PMID:14561886
7. ↑ Kim M, Krogan NJ, Vasiljeva L, Rando OJ, Nedea E, Greenblatt JF, Buratowski S. The yeast Rat1 exonuclease promotes transcription termination by RNA polymerase II. Nature. 2004 Nov 25;432(7016):517-22. PMID:15565157 doi:http://dx.doi.org/nature03041
8. ↑ Fang F, Phillips S, Butler JS. Rat1p and Rai1p function with the nuclear exosome in the processing and degradation of rRNA precursors. RNA. 2005 Oct;11(10):1571-8. PMID:16131592 doi:10.1261/rna.2900205
9. ↑ Luo W, Johnson AW, Bentley DL. The role of Rat1 in coupling mRNA 3'-end processing to transcription termination: implications for a unified allosteric-torpedo model. Genes Dev. 2006 Apr 15;20(8):954-65. PMID:16598041 doi:10.1101/gad.1409106
10. ↑ Stevens A, Poole TL. 5'-exonuclease-2 of Saccharomyces cerevisiae. Purification and features of ribonuclease activity with comparison to 5'-exonuclease-1. J Biol Chem. 1995 Jul 7;270(27):16063-9. PMID:7608167 doi:10.1074/jbc.270.27.16063
11. ↑ Kenna M, Stevens A, McCammon M, Douglas MG. An essential yeast gene with homology to the exonuclease-encoding XRN1/KEM1 gene also encodes a protein with exoribonuclease activity. Mol Cell Biol. 1993 Jan;13(1):341-50. PMID:8417335 doi:10.1128/mcb.13.1.341-350.1993
12. ↑ Poole TL, Stevens A. Structural modifications of RNA influence the 5' exoribonucleolytic hydrolysis by XRN1 and HKE1 of Saccharomyces cerevisiae. Biochem Biophys Res Commun. 1997 Jun 27;235(3):799-805. PMID:9207242 doi:10.1006/bbrc.1997.6877
13. ↑ Dichtl B, Stevens A, Tollervey D. Lithium toxicity in yeast is due to the inhibition of RNA processing enzymes. EMBO J. 1997 Dec 1;16(23):7184-95. PMID:9384595 doi:10.1093/emboj/16.23.7184
14. ↑ Petfalski E, Dandekar T, Henry Y, Tollervey D. Processing of the precursors to small nucleolar RNAs and rRNAs requires common components. Mol Cell Biol. 1998 Mar;18(3):1181-9. PMID:9488433
15. ↑ Villa T, Ceradini F, Presutti C, Bozzoni I. Processing of the intron-encoded U18 small nucleolar RNA in the yeast Mol Cell Biol. 1998 Jun;18(6):3376-83. PMID:9584178 doi:10.1128/MCB.18.6.3376
16. ↑ Qu LH, Henras A, Lu YJ, Zhou H, Zhou WX, Zhu YQ, Zhao J, Henry Y, Caizergues-Ferrer M, Bachellerie JP. Seven novel methylation guide small nucleolar RNAs are processed from a common polycistronic transcript by Rat1p and RNase III in yeast. Mol Cell Biol. 1999 Feb;19(2):1144-58. PMID:9891049 doi:10.1128/MCB.19.2.1144