1yo5

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spinqualitylabelsall models 1yo5, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Analysis of the 2.0A crystal structure of the protein-DNA complex of human PDEF Ets domain bound to the prostate specific antigen regulatory site

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

PDEF, a prostate epithelial specific transcription factor, is a member of, the Ets family of DNA binding proteins. Here we report a 2.0 A crystal, structure of the PDEF Ets domain in complex with a natural, high-affinity, DNA binding site in the promoter/enhancer region of the human prostate, specific antigen gene. Comparison of the PDEF-DNA complex with other Ets, complexes revealed key features that are shared among Ets members, as well, as important differences in substrate specification at both the "GGA" core, and the flanking regions of the DNA site. The combination of the serine, residue at position 308 and the glutamine at position 311 explains the, previous observation that the PDEF binds preferentially to a thymine at, the +4 position of its binding site. Despite the common essential features, that are shared among Ets members, PDEF demonstrates distinct patterns of, interactions at different positions of DNA in achieving sequence specific, recognition. Collectively, the common and unique interactions with both, the DNA bases and the backbone phosphates lead to substrate specificity, and individual preference for certain DNA sites.

Disease

Known diseases associated with this structure: Alzheimer disease, type 3 OMIM:[104311], Alzheimer disease, type 3, with spastic paraparesis and apraxia OMIM:[104311], Alzheimer disease, type 3, with spastic paraparesis and unusual plaques OMIM:[104311], Cardiomyopathy, dilated, 1U OMIM:[104311], Dementia, frontotemporal OMIM:[104311], Pick disease OMIM:[104311]

About this Structure

1YO5 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.

Reference

Analysis of the 2.0 A crystal structure of the protein-DNA complex of the human PDEF Ets domain bound to the prostate specific antigen regulatory site., Wang Y, Feng L, Said M, Balderman S, Fayazi Z, Liu Y, Ghosh D, Gulick AM, Biochemistry. 2005 May 17;44(19):7095-106. PMID:15882048

Page seeded by OCA on Mon Nov 12 20:22:10 2007