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Publication Abstract from PubMed

Nanobodies (VHHs) are single-domain antibodies with three antigenic CDR regions and are used in diverse scientific applications. Here, an approximately 14 kDa nanobody (A5) specific for the endonuclease VIII (Nei)-like 1 or NEIL1 DNA glycosylase involved in the first step of the base-excision repair pathway was crystallized and its structure was determined to 2.1 A resolution. The crystals posed challenges due to potential twinning and anisotropic diffraction. Despite inconclusive twinning indicators, reprocessing in an orthorhombic setting and molecular replacement in space group P2(1)2(1)2 enabled the successful modeling of 96% of residues in the asymmetric unit, with final R(work) and R(free) values of 0.199 and 0.229, respectively.

Deciphering the crystal structure of a novel nanobody against the NEIL1 DNA glycosylase.,Thompson MK, Sharma N, Thorn A, Prakash A Acta Crystallogr D Struct Biol. 2024 Feb 1;80(Pt 2):137-146. doi: , 10.1107/S205979832400038X. Epub 2024 Jan 30. PMID:38289715^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.