9fn9

Publication Abstract from PubMed

Nitroreductases are important enzymes for a variety of applications, including cancer therapy and bioremediation. They often require encapsulation to improve stability and activity. We focus on genetically encoded encapsulation of nitroreductases within protein capsids, like encapsulins. Our study showcases the encapsulation of nitroreductase NfsB as functional dimers within encapsulins, which enhances protein activity and stability in diverse conditions. Mutations within the pore region are beneficial for activity of the encapsulated enzyme, potentially by increasing diffusion rates. Cryogenic electron microscopy reveals the overall architecture of the encapsulated dimeric NfsB within the nanoreactor environment and identifies multiple pore states in the shell. These findings highlight the potential of encapsulins as versatile tools for enhancing enzyme performance across various fields.

A nanoengineered tandem nitroreductase: designing a robust prodrug-activating nanoreactor.,Zmyslia M, Capper MJ, Grimmeisen M, Sartory K, Deuringer B, Abdelsalam M, Shen K, Jung M, Sippl W, Koch HG, Kaul L, Suss R, Kohnke J, Jessen-Trefzer C RSC Chem Biol. 2024 Nov 4;6(1):21-35. doi: 10.1039/d4cb00127c. PMID:39508026^[1]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.