1ywh

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spinqualitylabelsall models 1ywh, resolution 2.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

crystal structure of urokinase plasminogen activator receptor

Overview

We report the crystal structure of a soluble form of human urokinase-type, plasminogen activator receptor (uPAR/CD87), which is expressed at the, invasive areas of the tumor-stromal microenvironment in many human, cancers. The structure was solved at 2.7 A in association with a, competitive peptide inhibitor of the urokinase-type plasminogen activator, (uPA)-uPAR interaction. uPAR is composed of three consecutive three-finger, domains organized in an almost circular manner, which generates both a, deep internal cavity where the peptide binds in a helical conformation, and a large external surface. This knowledge combined with the discovery, of a convergent binding motif shared by the antagonist peptide and uPA, allowed us to build a model of the human uPA-uPAR complex. This model, reveals that the receptor-binding module of uPA engages the uPAR central, cavity, thus leaving the external receptor surface accessible for other, protein interactions (vitronectin and integrins). By this unique, structural assembly, uPAR can orchestrate the fine interplay with the, partners that are required to guide uPA-focalized proteolysis on the cell, surface and control cell adhesion and migration.

About this Structure

1YWH is a Single protein structure of sequence from Homo sapiens with NDG, NAG and SO4 as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of the human urokinase plasminogen activator receptor bound to an antagonist peptide., Llinas P, Le Du MH, Gardsvoll H, Dano K, Ploug M, Gilquin B, Stura EA, Menez A, EMBO J. 2005 May 4;24(9):1655-63. Epub 2005 Apr 7. PMID:15861141

Page seeded by OCA on Mon Nov 12 20:25:40 2007