DNA repair protein

Function

DNA repair proteins correct DNA damage caused by mutations or toxins^[1].

• DNA repair protein Rad4 is a yeast protein involved in DNA damage recognition in nucleotide excision repair^[2]. Rad4 is an ortholog of the XPC protein. Rad4 opens up damaged DNA by inserting β-hairpin into the duplex and flipping out two damage-containing nucleotide pairs^[3].
• DNA repair protein Rev1 is a DNA polymerase active in DNA damage sites. It contains 2 ubiquitin-binding sites^[4].

Disease

Mutations in DNA repair protein Rad4 can cause the xeroderma pigmentosum skin cancer predisposition syndrome^[5].

Structural highlights

(PDB entry 2qsg). inserts into DNA making hydrogen bond interactions as well as non-bonding interactions with it^[6]. .

3D Structures of DNA repair protein

DNA repair protein 3D structures

References

1. ↑ Tainer JA, Thayer MM, Cunningham RP. DNA repair proteins. Curr Opin Struct Biol. 1995 Feb;5(1):20-6. PMID:7773744 doi:10.1016/0959-440x(95)80005-l
2. ↑ den Dulk B, Brandsma JA, Brouwer J. The Rad4 homologue YDR314C is essential for strand-specific repair of RNA polymerase I-transcribed rDNA in Saccharomyces cerevisiae. Mol Microbiol. 2005 Jun;56(6):1518-26. doi: 10.1111/j.1365-2958.2005.04607.x. PMID:15916602 doi:http://dx.doi.org/10.1111/j.1365-2958.2005.04607.x
3. ↑ Velmurugu Y, Chen X, Slogoff Sevilla P, Min JH, Ansari A. Twist-open mechanism of DNA damage recognition by the Rad4/XPC nucleotide excision repair complex. Proc Natl Acad Sci U S A. 2016 Apr 19;113(16):E2296-305. doi:, 10.1073/pnas.1514666113. Epub 2016 Mar 31. PMID:27035942 doi:http://dx.doi.org/10.1073/pnas.1514666113
4. ↑ Cui G, Botuyan MV, Mer G. Structural Basis for the Interaction of Mutasome Assembly Factor REV1 with Ubiquitin. J Mol Biol. 2018 Jul 6;430(14):2042-2050. doi: 10.1016/j.jmb.2018.05.017. Epub, 2018 May 18. PMID:29778604 doi:http://dx.doi.org/10.1016/j.jmb.2018.05.017
5. ↑ Min JH, Pavletich NP. Recognition of DNA damage by the Rad4 nucleotide excision repair protein. Nature. 2007 Oct 4;449(7162):570-5. Epub 2007 Sep 19. PMID:17882165 doi:10.1038/nature06155
6. ↑ Min JH, Pavletich NP. Recognition of DNA damage by the Rad4 nucleotide excision repair protein. Nature. 2007 Oct 4;449(7162):570-5. Epub 2007 Sep 19. PMID:17882165 doi:10.1038/nature06155