JMS/Sandbox/msn2

From Proteopedia

Interactive 3-D Model

<jmolLink>
 <script>
  load $DATA/AF-P33748-F1-model_v4.pdb;  # AlphaFold prediction :contentReference[oaicite:0]{index=0}:contentReference[oaicite:1]{index=1}
  select protein; cartoon on; color chain; wireframe off; spacefill off;
  zoom 1.3;
 </script>
 <text>Initial full-length model</text>
</jmolLink>

• Full-length model (restore the starting view)
• Zinc finger DNA-binding domain
• Regulated nuclear-localization signal (NLS)
• PKA phosphorylation cluster
• C2H2 zinc-binding motif
• Intrinsically disordered regions (IDRs)
• Confidence coloring (pLDDT)

Quick Guide

• **C-terminal zinc finger (645-704)** binds STRE/G-quadruplex DNA.
• **Regulated NLS (618-633)** is masked by **PKA phosphorylation** (S582, S620, S625, S633) to control stress-dependent nuclear import.
• Most of the N-terminal 600 aa are **intrinsically disordered**, housing the NES and trans-activation motifs.
• Per-residue **pLDDT** values (confidence coloring scene) highlight the single well-folded domain vs. the flexible IDRs.

AlphaFold PDB header and full sequence: :contentReference[oaicite:2]{index=2}:contentReference[oaicite:3]{index=3}