| Structural highlights
Function
MCAPS_TYYVF Minor component of the viral capsid involved in aphid transmission and virus accumulation in the host (PubMed:10627524, PubMed:20416918, PubMed:26402374, PubMed:7882968). Required for the virus to move through the aphid (By similarity). The RTD domain of the protein is exposed on the surface of the particle and determines the vector specificity and intestinal tropism in the aphid (PubMed:7882968).[UniProtKB:P09516][1] [2] [3] [4]
Publication Abstract from PubMed
Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP *) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP * contains the CP sequence fused with a structured domain, denoted (N)RTD, which is a key determinant of virus transmission. Though both CP and RTP * are involved in virus movement and aphid transmission, how RTP * is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP * does not impair the capsid structure, and the (N)RTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP *s suggests a random insertion.
Structure of the turnip yellows virus particles.,Lai-Kee-Him J, Trapani S, Boissinot S, Reinbold C, Fallet C, Ancelin A, Lecorre F, Hoh F, Ziegler-Graff V, Brault V, Bron P Virology. 2025 Jun;607:110514. doi: 10.1016/j.virol.2025.110514. Epub 2025 Mar , 26. PMID:40179450[5]
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.
References
- ↑ Brault V, Mutterer J, Scheidecker D, Simonis MT, Herrbach E, Richards K, Ziegler-Graff V. Effects of point mutations in the readthrough domain of the beet western yellows virus minor capsid protein on virus accumulation in planta and on transmission by aphids. J Virol. 2000 Feb;74(3):1140-8. doi: 10.1128/jvi.74.3.1140-1148.2000. PMID:10627524 doi:http://dx.doi.org/10.1128/jvi.74.3.1140-1148.2000
- ↑ Revollon S, Strub JM, Fitchette AC, Wiss L, Gomord V, Van Dorsselaer A, Brault V. A reinvestigation provides no evidence for sugar residues on structural proteins of poleroviruses and argues against a role for glycosylation of virus structural proteins in aphid transmission. Virology. 2010 Jul 5;402(2):303-14. doi: 10.1016/j.virol.2010.03.037. Epub 2010, Apr 22. PMID:20416918 doi:http://dx.doi.org/10.1016/j.virol.2010.03.037
- ↑ Rodriguez-Medina C, Boissinot S, Chapuis S, Gereige D, Rastegar M, Erdinger M, Revers F, Ziegler-Graff V, Brault V. A protein kinase binds the C-terminal domain of the readthrough protein of Turnip yellows virus and regulates virus accumulation. Virology. 2015 Dec;486:44-53. doi: 10.1016/j.virol.2015.08.031. Epub 2015 Sep 21. PMID:26402374 doi:http://dx.doi.org/10.1016/j.virol.2015.08.031
- ↑ Brault V, van den Heuvel JF, Verbeek M, Ziegler-Graff V, Reutenauer A, Herrbach E, Garaud JC, Guilley H, Richards K, Jonard G. Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74. EMBO J. 1995 Feb 15;14(4):650-9. doi: 10.1002/j.1460-2075.1995.tb07043.x. PMID:7882968 doi:http://dx.doi.org/10.1002/j.1460-2075.1995.tb07043.x
- ↑ Lai-Kee-Him J, Trapani S, Boissinot S, Reinbold C, Fallet C, Ancelin A, Lecorre F, Hoh F, Ziegler-Graff V, Brault V, Bron P. Structure of the turnip yellows virus particles. Virology. 2025 Jun;607:110514. PMID:40179450 doi:10.1016/j.virol.2025.110514
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