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Publication Abstract from PubMed

The biologically active components of Conus venoms are mainly small peptides with disulfide-bonded structures. Some conotoxins contain post-translational modifications as an evolutionary strategy to enhance their potency and selectivity towards ion channels and receptors. Few conotoxins are known to target mammalian Kv1 channels. kappa-SrXIA from the venom of Conus spurius inhibits the voltage-gated potassium Kv1.2 and Kv1.6 channels through a basic ring of Arg. The 32 amino acid kappa-SrXIA has eight Cys residues arranged in the pattern that defines the I-superfamily with four disulfide bridges, two gamma-carboxy-glutamates (Gla), and a Pro-amidated C-terminus (X). In this study, we obtained and determined the biological activity and NMR structure of recombinant kappa-SrXIA without Gla and X. The conotoxin cDNA was expressed in E. coli CD41 and purified by GST-affinity chromatography and RP-HPLC. Pharmacological assays were performed by two-electrode voltage-clamp recordings in Xenopus laevis oocytes expressing recombinant Kv1.1, Kv1.2, Kv1.3, Kv1.4 and Kv1.6 channels. Except for Kv1.3, kappa-Sr-XIA irreversibly blocked Kv1 channels displaying a lower affinity and a slower inhibition kinetics than native conotoxin. Even when kappa-SrXIA displayed a high structural similarity to GXIA from the I(3)-superfamily, the resultant disulfide connectivity forming an ICK+1 motif showed two antiparallel beta-strands as iota-RXIA from the I(1)-superfamily. This study represents the first 3D NMR structure for a member of the I(2)-superfamily.

Block of Kv1 potassium channels and NMR structure of recombinant conotoxin kappa-SrXIA.,Escobar LI, Quezada Suaste CD, Salvador C, Aparicio DL, Melchor-Meneses CM, Bravo-Martinez J, de la Rosa V, Lopez-Gonzalez Z, Del Rio-Portilla F Toxicon. 2025 May 3;262:108384. doi: 10.1016/j.toxicon.2025.108384. PMID:40324600^[3]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine.