| Structural highlights
Function
SPG1_STRSG Binds to the constant Fc region of IgG with high affinity.CSOCA_HALNC Reversible hydration of carbon dioxide (PubMed:14729686, PubMed:17012396, PubMed:18258595). Essential for chemolithotrophic carbon dioxide fixation, supplies CO(2) to RuBisCO (ribulose bisphosphate carboxylase, cbbL-cbbS) in the carboxysome (Probable) (PubMed:18258595). There are estimated to be 40 CsoSCA dimers per carboxysome (Ref.5).[1] [2] [3] [4] [5] Unlike beta-carboxysomes, alpha-carboxysomes (Cb) can form without cargo protein. CsoS2 is essential for Cb formation and is also capable of targeting foreign proteins to the Cb. The Cb shell assembles with the aid of CsoS2; CsoS1A, CsoS1B and CsoS1C form the majority of the shell while CsoS4A and CsoS4B form vertices. CsoS1D forms pseudohexamers that probably control metabolite flux into and out of the shell.[6]
References
- ↑ So AK, Espie GS, Williams EB, Shively JM, Heinhorst S, Cannon GC. A novel evolutionary lineage of carbonic anhydrase (epsilon class) is a component of the carboxysome shell. J Bacteriol. 2004 Feb;186(3):623-30. PMID:14729686 doi:10.1128/JB.186.3.623-630.2004
- ↑ Heinhorst S, Williams EB, Cai F, Murin CD, Shively JM, Cannon GC. Characterization of the carboxysomal carbonic anhydrase CsoSCA from Halothiobacillus neapolitanus. J Bacteriol. 2006 Dec;188(23):8087-94. PMID:17012396 doi:10.1128/JB.00990-06
- ↑ Dou Z, Heinhorst S, Williams EB, Murin CD, Shively JM, Cannon GC. CO2 fixation kinetics of Halothiobacillus neapolitanus mutant carboxysomes lacking carbonic anhydrase suggest the shell acts as a diffusional barrier for CO2. J Biol Chem. 2008 Apr 18;283(16):10377-84. PMID:18258595 doi:10.1074/jbc.M709285200
- ↑ Dou Z, Heinhorst S, Williams EB, Murin CD, Shively JM, Cannon GC. CO2 fixation kinetics of Halothiobacillus neapolitanus mutant carboxysomes lacking carbonic anhydrase suggest the shell acts as a diffusional barrier for CO2. J Biol Chem. 2008 Apr 18;283(16):10377-84. PMID:18258595 doi:10.1074/jbc.M709285200
- ↑ So AK, Espie GS, Williams EB, Shively JM, Heinhorst S, Cannon GC. A novel evolutionary lineage of carbonic anhydrase (epsilon class) is a component of the carboxysome shell. J Bacteriol. 2004 Feb;186(3):623-30. PMID:14729686 doi:10.1128/JB.186.3.623-630.2004
- ↑ Li T, Jiang Q, Huang J, Aitchison CM, Huang F, Yang M, Dykes GF, He HL, Wang Q, Sprick RS, Cooper AI, Liu LN. Reprogramming bacterial protein organelles as a nanoreactor for hydrogen production. Nat Commun. 2020 Oct 28;11(1):5448. doi: 10.1038/s41467-020-19280-0. PMID:33116131 doi:http://dx.doi.org/10.1038/s41467-020-19280-0
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