1wbp
From Proteopedia
SRPK1 BOUND TO 9MER DOCKING MOTIF PEPTIDE
Overview
The arginine-serine (RS)-rich domain of the SR protein ASF/SF2 is phosphorylated by SR protein kinases (SRPKs) and Clk/Sty kinases. However, the mode of phosphorylation by these kinases and their coordination in the biological regulation of ASF/SF2 is unknown. Here, we report the crystal structure of an active fragment of human SRPK1 bound to a peptide derived from an SR protein. This structure led us to identify a docking motif in ASF/SF2. We find that this docking motif restricts phosphorylation of ASF/SF2 by SRPK1 to the N-terminal part of the RS domain - a property essential for its assembly into nuclear speckles. We further show that Clk/Sty causes release of ASF/SF2 from speckles by phosphorylating the C-terminal part of its RS domain. These results suggest that the docking motif of ASF/SF2 is a key regulatory element for sequential phosphorylation by SRPK1 and Clk/Sty and, thus, is essential for its subcellular localization.
About this Structure
1WBP is a Protein complex structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Interplay between SRPK and Clk/Sty kinases in phosphorylation of the splicing factor ASF/SF2 is regulated by a docking motif in ASF/SF2., Ngo JC, Chakrabarti S, Ding JH, Velazquez-Dones A, Nolen B, Aubol BE, Adams JA, Fu XD, Ghosh G, Mol Cell. 2005 Oct 7;20(1):77-89. PMID:16209947 Page seeded by OCA on Sat May 3 13:25:52 2008
Categories: Homo sapiens | Non-specific serine/threonine protein kinase | Protein complex | Adams, J A. | Aubol, B E. | Chakrabarti, S. | Ding, J H. | Fu, X D. | Ghosh, G. | Ngo, J C. | Nolen, B. | Velazquez-Dones, A. | Alternative splicing | Atp-binding | Chromosome partition | Differentiation | Kinase | Mrna processing | Mrna splicing | Nuclear protein | Nucleotide-binding | Phosphorylation | Serine/threonine-protein kinase | Srpk | Transferase
