1gmz
From Proteopedia
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CRYSTAL STRUCTURE OF THE D49 PHOSPHOLIPASE A2 PIRATOXIN III FROM BOTHROPS PIRAJAI.
Overview
Snake venoms are rich sources of phospholipase A(2) homologues, both, active calcium-binding Asp49 enzymes and essentially inactive Lys49, proteins. They are responsible for multiple pharmacological effects, some, of which are dependent on catalytic activity and others of which are not., Here, the 2.4 A X-ray crystal structure of an active Asp49 phospholipase, A(2) from the venom of the snake Bothrops pirajai, refined to conventional, and free R values of 20.1 and 25.5%, respectively, is reported. Unusually, for phospholipases A(2), the dependence of the enzyme rate on the, substrate concentration is sigmoidal, implying cooperativity of substrate, binding. The unprecedented structural distortion seen for the, calcium-binding loop in the present structure may therefore be indicative, of a ... [(full description)]
About this Structure
1GMZ is a [Single protein] structure of sequence from [Bothrops pirajai] with IPA as [ligand]. Active as [[1]], with EC number [3.1.1.4]. Full crystallographic information is available from [OCA].
Reference
The structure of the D49 phospholipase A2 piratoxin III from Bothrops pirajai reveals unprecedented structural displacement of the calcium-binding loop: possiblerelationship to cooperative substrate binding., Rigden DJ, Hwa LW, Marangoni S, Toyama MH, Polikarpov I, Acta Crystallogr D Biol Crystallogr. 2003 Feb;59(Pt 2):255-62. Epub 2003, Jan 23. PMID:12554936
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