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2ayo
From Proteopedia
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Structure of USP14 bound to ubquitin aldehyde
Overview
The ubiquitin-specific processing protease (UBP) family of, deubiquitinating enzymes plays an essential role in numerous cellular, processes. Mammalian USP14 (Ubp6 in yeast) is unique among known UBP, enzymes in that it is activated catalytically upon specific association, with the 26S proteasome. Here, we report the crystal structures of the, 45-kDa catalytic domain of USP14 in isolation and in a complex with, ubiquitin aldehyde, which reveal distinct structural features. In the, absence of ubiquitin binding, the catalytic cleft leading to the active, site of USP14 is blocked by two surface loops. Binding by ubiquitin, induces a significant conformational change that translocates the two, surface loops thereby allowing access of the ubiquitin C-terminus to the, active site. These structural observations, in conjunction with, biochemical characterization, identify important regulatory mechanisms for, USP14.
About this Structure
2AYO is a Protein complex structure of sequences from Homo sapiens. Active as Ubiquitin thiolesterase, with EC number 3.1.2.15 Full crystallographic information is available from OCA.
Reference
Structure and mechanisms of the proteasome-associated deubiquitinating enzyme USP14., Hu M, Li P, Song L, Jeffrey PD, Chenova TA, Wilkinson KD, Cohen RE, Shi Y, EMBO J. 2005 Nov 2;24(21):3747-56. Epub 2005 Oct 6. PMID:16211010
Page seeded by OCA on Mon Nov 12 20:56:16 2007
