2brq

From Proteopedia

proteopedia linkproteopedia link

spinqualitylabelsall models 2brq, resolution 2.10Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

CRYSTAL STRUCTURE OF THE FILAMIN A REPEAT 21 COMPLEXED WITH THE INTEGRIN BETA7 CYTOPLASMIC TAIL PEPTIDE

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The ability of adhesion receptors to transmit biochemical signals and, mechanical force across cell membranes depends on interactions with the, actin cytoskeleton. Filamins are large, actin-crosslinking proteins that, connect multiple transmembrane and signaling proteins to the cytoskeleton., Here, we describe the high-resolution structure of an interface between, filamin A and an integrin adhesion receptor. When bound, the integrin beta, cytoplasmic tail forms an extended beta strand that interacts with beta, strands C and D of the filamin immunoglobulin-like domain (IgFLN) 21. This, interface is common to many integrins, and we suggest it is a prototype, for other IgFLN domain interactions. Notably, the structurally defined, filamin binding site overlaps with that of the integrin-regulator talin, and these proteins compete for binding to integrin tails, allowing, integrin-filamin interactions to impact talin-dependent integrin, activation. Phosphothreonine-mimicking mutations inhibit filamin, but not, talin, binding, indicating that kinases may modulate this competition and, provide additional means to control integrin functions.

Disease

Known diseases associated with this structure: Frontometaphyseal dysplasia OMIM:[300017], Heterotopia, periventricular OMIM:[300017], Heterotopia, periventricular nodular, with frontometaphyseal dysplasia OMIM:[300017], Heterotopia, periventricular, ED variant OMIM:[300017], Melnick-Needles syndrome OMIM:[300017], Otopalatodigital syndrome, type I OMIM:[300017], Otopalatodigital syndrome, type II OMIM:[300017]

About this Structure

2BRQ is a Protein complex structure of sequences from Homo sapiens with GTT and GOL as ligands. Structure known Active Site: AC1. Full crystallographic information is available from OCA.

Reference

The molecular basis of filamin binding to integrins and competition with talin., Kiema T, Lad Y, Jiang P, Oxley CL, Baldassarre M, Wegener KL, Campbell ID, Ylanne J, Calderwood DA, Mol Cell. 2006 Feb 3;21(3):337-47. PMID:16455489

Page seeded by OCA on Mon Nov 12 21:05:45 2007