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2c9a
From Proteopedia
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CRYSTAL STRUCTURE OF THE MAM-IG MODULE OF RECEPTOR PROTEIN TYROSINE PHOSPHATASE MU
Overview
Type IIB receptor protein tyrosine phosphatases (RPTPs) are bi-functional, cell surface molecules. Their ectodomains mediate stable, homophilic, cell-adhesive interactions, whereas the intracellular catalytic regions, can modulate the phosphorylation state of cadherin/catenin complexes. We, describe a systematic investigation of the cell-adhesive properties of the, extracellular region of RPTPmu, a prototypical type IIB RPTP. The crystal, structure of a construct comprising its N-terminal MAM (meprin/A5/mu) and, Ig domains was determined at 2.7 A resolution; this assigns the MAM fold, to the jelly-roll family and reveals extensive interactions between the, two domains, which form a rigid structural unit. Structure-based, site-directed mutagenesis, serial domain deletions and cell-adhesion, assays allowed us to identify the four N-terminal domains (MAM, Ig, fibronectin type III (FNIII)-1 and FNIII-2) as a minimal functional unit., Biophysical characterization revealed at least two independent types of, homophilic interaction which, taken together, suggest that there is the, potential for formation of a complex and possibly ordered array of, receptor molecules at cell contact sites.
About this Structure
2C9A is a Single protein structure of sequence from Homo sapiens with NAG and NA as ligands. Active as Protein-tyrosine-phosphatase, with EC number 3.1.3.48 Structure known Active Site: AC1. Full crystallographic information is available from OCA.
Reference
Molecular analysis of receptor protein tyrosine phosphatase mu-mediated cell adhesion., Aricescu AR, Hon WC, Siebold C, Lu W, van der Merwe PA, Jones EY, EMBO J. 2006 Feb 22;25(4):701-12. Epub 2006 Feb 2. PMID:16456543
Page seeded by OCA on Mon Nov 12 21:13:00 2007
