1z9e
From Proteopedia
Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75
Overview
Lens epithelium-derived growth factor (LEDGF)/p75 is the dominant binding partner of HIV-1 integrase (IN) in human cells. We have determined the NMR structure of the integrase-binding domain (IBD) in LEDGF and identified amino acid residues essential for the interaction. The IBD is a compact right-handed bundle composed of five alpha-helices. Based on folding topology, the IBD is structurally related to a diverse family of alpha-helical proteins that includes eukaryotic translation initiation factor eIF4G and karyopherin-beta. LEDGF residues essential for the interaction with IN were localized to interhelical loop regions of the bundle structure. Interaction-defective IN mutants were previously shown to cripple replication although they retained catalytic function. The initial structure determination of a host cell factor that tightly binds to a retroviral enzyme lays the groundwork for understanding enzyme-host interactions important for viral replication.
About this Structure
1Z9E is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Solution structure of the HIV-1 integrase-binding domain in LEDGF/p75., Cherepanov P, Sun ZY, Rahman S, Maertens G, Wagner G, Engelman A, Nat Struct Mol Biol. 2005 Jun;12(6):526-32. Epub 2005 May 15. PMID:15895093 Page seeded by OCA on Sat May 3 17:20:49 2008
