2err
From Proteopedia
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NMR Structure of the RNA Binding Domain of Human Fox-1 in Complex with UGCAUGU
Overview
The Fox-1 protein regulates alternative splicing of tissue-specific exons, by binding to GCAUG elements. Here, we report the solution structure of, the Fox-1 RNA binding domain (RBD) in complex with UGCAUGU. The last three, nucleotides, UGU, are recognized in a canonical way by the four-stranded, beta-sheet of the RBD. In contrast, the first four nucleotides, UGCA, are, bound by two loops of the protein in an unprecedented manner. Nucleotides, U1, G2, and C3 are wrapped around a single phenylalanine, while G2 and A4, form a base-pair. This novel RNA binding site is independent from the, beta-sheet binding interface. Surface plasmon resonance analyses were used, to quantify the energetic contributions of electrostatic and hydrogen bond, interactions to complex formation and support our structural findings., These results demonstrate the unusual molecular mechanism of, sequence-specific RNA recognition by Fox-1, which is exceptional in its, high affinity for a defined but short sequence element.
About this Structure
2ERR is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Molecular basis of RNA recognition by the human alternative splicing factor Fox-1., Auweter SD, Fasan R, Reymond L, Underwood JG, Black DL, Pitsch S, Allain FH, EMBO J. 2006 Jan 11;25(1):163-73. Epub 2005 Dec 15. PMID:16362037
Page seeded by OCA on Mon Nov 12 21:54:41 2007
