2evz
From Proteopedia
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Structure of RNA Binding Domains 3 and 4 of Polypyrimidine Tract Binding Protein
Overview
The polypyrimidine tract binding protein (PTB) is a 58 kDa protein, involved in many aspects of RNA metabolism. In this study, we focused our, attention on the structure of the two C-terminal RNA recognition motifs, (RRM3 and RRM4) of PTB. In a previous study, it was found that the two, RRMs are independent in the free state. We recently determined the, structure of the same fragment in complex with RNA and found that the two, RRMs interact extensively. This difference made us re-evaluate in detail, the free protein structure and in particular the interdomain interface. We, used a combination of NMR spectroscopy and segmental isotopic labeling to, unambiguously study and characterize the interdomain interactions. An, improved segmental isotopic labeling protocol was used, enabling us to, unambiguously identify 130 interdomain NOEs between the two RRMs and to, calculate a very precise structure. The structure reveals a large, interdomain interface, resulting in a very unusual positioning of the two, RRM domains relative to one another.
About this Structure
2EVZ is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Structure of the two most C-terminal RNA recognition motifs of PTB using segmental isotope labeling., Vitali F, Henning A, Oberstrass FC, Hargous Y, Auweter SD, Erat M, Allain FH, EMBO J. 2006 Jan 11;25(1):150-62. Epub 2005 Dec 15. PMID:16362043
Page seeded by OCA on Mon Nov 12 21:56:30 2007
