2ezd
From Proteopedia
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SOLUTION STRUCTURE OF A COMPLEX OF THE SECOND DNA BINDING DOMAIN OF HUMAN HMG-I(Y) BOUND TO DNA DODECAMER CONTAINING THE PRDII SITE OF THE INTERFERON-BETA PROMOTER, NMR, MINIMIZED AVERAGE STRUCTURE
Contents |
Overview
The solution structure of a complex between a truncated form of HMG-I(Y), consisting of the second and third DNA binding domains (residues 51-90), and a DNA dodecamer containing the PRDII site of the interferon-beta, promoter has been solved by multidimensional nuclear magnetic resonance, spectroscopy. The stoichiometry of the complex is one molecule of HMG-I(Y), to two molecules of DNA. The structure reveals a new architectural minor, groove binding motif which stabilizes B-DNA, thereby facilitating the, binding of other transcription factors in the opposing major groove. The, interactions involve a central Arg-Gly-Arg motif together with two other, modules that participate in extensive hydrophobic and polar contracts. The, absence of one of these modules in the third DNA binding domain accounts, for its-100 fold reduced affinity relative to the second one.
Disease
Known diseases associated with this structure: Lipoma (1) OMIM:[600701]
About this Structure
2EZD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
The solution structure of an HMG-I(Y)-DNA complex defines a new architectural minor groove binding motif., Huth JR, Bewley CA, Nissen MS, Evans JN, Reeves R, Gronenborn AM, Clore GM, Nat Struct Biol. 1997 Aug;4(8):657-65. PMID:9253416
Page seeded by OCA on Mon Nov 12 21:57:34 2007
