2f8v

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spinqualitylabelsall models 2f8v, resolution 2.75Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Structure of full length telethonin in complex with the N-terminus of titin

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

The Z-disk region defines the lateral boundary of the sarcomere and, requires a high level of mechanical strength to provide a stable framework, for large filamentous muscle proteins. The level of complexity at this, area is reflected by a large number of protein-protein interactions., Recently, we unraveled how the N-terminus of the longest filament, component, the giant muscle protein titin, is assembled into an, antiparallel (2:1) sandwich complex by the N-terminal titin-binding, segment of the Z-disk ligand telethonin/T-cap [Zou, P., Pinotsis, N., Lange, S., Song, Y.H., Popov, A., Mavridis, I., Mayans, O.M., Gautel, M., Wilmanns, M., 2006. Palindromic assembly of the giant muscle protein titin, in the sarcomeric Z-disk. Nature 439, 229-233]. In this contribution, we, present structural data of a related complex of the titin N-terminus with, full-length telethonin. The C-terminus of telethonin remains invisible, suggesting that it does not fold into a defined structure even in the, presence of titin. In contrast to the structure with truncated telethonin, a dimer of two titin/telethonin complexes is formed within the crystal, environment, potentially indicating the formation of higher oligomers. We, further investigated the structure and dynamics of this assembly by, small-angle X-ray scattering, circular dichroism, and in vivo, complementation data. The data consistently indicate the involvement of, the C-terminal part of telethonin into the assembly of two, titin/telethonin complexes.

Disease

Known diseases associated with this structure: Cardiomyopathy, dilated, 1G OMIM:[188840], Cardiomyopathy, dilated, 1N OMIM:[604488], Cardiomyopathy, familial hypertrophic OMIM:[188840], Muscular dystrophy, limb-girdle, type 2G OMIM:[604488], Muscular dystrophy, limb-girdle, type 2J OMIM:[188840], Myopathy, proximal, with early respiratory muscle involvement OMIM:[188840], Tibial muscular dystrophy, tardive OMIM:[188840]

About this Structure

2F8V is a Protein complex structure of sequences from Homo sapiens with SO4 as ligand. Full crystallographic information is available from OCA.

Reference

Evidence for a dimeric assembly of two titin/telethonin complexes induced by the telethonin C-terminus., Pinotsis N, Petoukhov M, Lange S, Svergun D, Zou P, Gautel M, Wilmanns M, J Struct Biol. 2006 Aug;155(2):239-50. Epub 2006 Apr 27. PMID:16713295

Page seeded by OCA on Mon Nov 12 22:01:24 2007