2gli

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spinqualitylabelsall models 2gli, resolution 2.600Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

FIVE-FINGER GLI/DNA COMPLEX

Contents 1 Overview 2 Disease 3 About this Structure 4 Reference

Overview

Zinc finger proteins, of the type first discovered in transcription factor, IIIA (TFIIIA), are one of the largest and most important families of, DNA-binding proteins. The crystal structure of a complex containing the, five Zn fingers from the human GLI oncogene and a high-affinity DNA, binding site has been determined at 2.6 A resolution. Finger one does not, contact the DNA. Fingers two through five bind in the major groove and, wrap around the DNA, but lack the simple, strictly periodic arrangement, observed in the Zif268 complex. Fingers four and five of GLI make, extensive base contacts in a conserved nine base-pair region, and this, section of the DNA has a conformation intermediate between B-DNA and, A-DNA. Analyzing the GLI complex and comparing it with Zif268 offers new, perspectives on Zn finger-DNA recognition.

Disease

Known diseases associated with this structure: Holoprosencephaly-9 OMIM:[165230], Pituitary anomalies with holoprosencephaly-like features OMIM:[165230]

About this Structure

2GLI is a Single protein structure of sequence from Homo sapiens with CO as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of a five-finger GLI-DNA complex: new perspectives on zinc fingers., Pavletich NP, Pabo CO, Science. 1993 Sep 24;261(5129):1701-7. PMID:8378770

Page seeded by OCA on Mon Nov 12 22:19:58 2007