2d3m
From Proteopedia
Pentaketide chromone synthase complexed with coenzyme A
Overview
The crystal structures of a wild-type and a mutant PCS, a novel plant type III polyketide synthase from a medicinal plant, Aloe arborescens, were solved at 1.6 A resolution. The crystal structures revealed that the pentaketide-producing wild-type and the octaketide-producing M207G mutant shared almost the same overall folding, and that the large-to-small substitution dramatically increases the volume of the polyketide-elongation tunnel by opening a gate to two hidden pockets behind the active site of the enzyme. The chemically inert active site residue 207 thus controls the number of condensations of malonyl-CoA, solely depending on the steric bulk of the side chain. These findings not only provided insight into the polyketide formation reaction, but they also suggested strategies for the engineered biosynthesis of polyketides.
About this Structure
2D3M is a Single protein structure of sequence from Aloe arborescens. Full crystallographic information is available from OCA.
Reference
Structural insight into chain-length control and product specificity of pentaketide chromone synthase from Aloe arborescens., Morita H, Kondo S, Oguro S, Noguchi H, Sugio S, Abe I, Kohno T, Chem Biol. 2007 Apr;14(4):359-69. PMID:17462571 Page seeded by OCA on Sat May 3 23:38:33 2008
