2har
From Proteopedia
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Crystal structure of VDR LBD in complex with 2 alpha-(3-hydroxy-1-propoxy) calcitriol
Contents |
Overview
The crystal structure of the vitamin D receptor (VDR) in complex with 1, alpha,25(OH)2D3 revealed the presence of several water molecules near the, A-ring linking the ligand C-2 position to the protein surface. Here, we, report the crystal structures of the human VDR ligand binding domain bound, to selected C-2 alpha substituted analogues, namely, methyl, propyl, propoxy, hydroxypropyl, and hydroxypropoxy. These specific replacements do, not modify the structure of the protein or the ligand, but with the, exception of the methyl substituent, all analogues affect the presence, and/or the location of the above water molecules. The integrity of the, channel interactions and specific C-2 alpha analogue directed additional, interactions correlate with the binding affinity of the ligands. In, contrast, the resulting loss or gain of H-bonds does not reflect the, magnitude of HL60 cell differentiation. Our overall findings highlight a, rational approach to the design of more potent ligands by building in, features revealed in the crystal structures.
Disease
Known diseases associated with this structure: Osteoporosis, involutional, 166710 (1) OMIM:[601769], Rickets, vitamin D-resistant, type IIA OMIM:[601769]
About this Structure
2HAR is a Single protein structure of sequence from Homo sapiens with OCC as ligand. Full crystallographic information is available from OCA.
Reference
Probing a water channel near the A-ring of receptor-bound 1 alpha,25-dihydroxyvitamin D3 with selected 2 alpha-substituted analogues., Hourai S, Fujishima T, Kittaka A, Suhara Y, Takayama H, Rochel N, Moras D, J Med Chem. 2006 Aug 24;49(17):5199-205. PMID:16913708
Page seeded by OCA on Mon Nov 12 22:29:12 2007
