2e5l
From Proteopedia
A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine- Dalgarno interaction
Overview
In the initiation phase of bacterial translation, the 30S ribosomal subunit captures mRNA in preparation for binding with initiator tRNA. The purine-rich Shine-Dalgarno (SD) sequence, in the 5' untranslated region of the mRNA, anchors the 30S subunit near the start codon, via base pairing with an anti-SD (aSD) sequence at the 3' terminus of 16S rRNA. Here, we present the 3.3 A crystal structure of the Thermus thermophilus 30S subunit bound with an mRNA mimic. The duplex formed by the SD and aSD sequences is snugly docked in a "chamber" between the head and platform domains, demonstrating how the 30S subunit captures and stabilizes the otherwise labile SD helix. This location of the SD helix is suitable for the placement of the start codon AUG in the immediate vicinity of the mRNA channel, in agreement with reported crosslinks between the second position of the start codon and G1530 of 16S rRNA.
About this Structure
2E5L is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.
Reference
A snapshot of the 30S ribosomal subunit capturing mRNA via the Shine-Dalgarno interaction., Kaminishi T, Wilson DN, Takemoto C, Harms JM, Kawazoe M, Schluenzen F, Hanawa-Suetsugu K, Shirouzu M, Fucini P, Yokoyama S, Structure. 2007 Mar;15(3):289-97. PMID:17355865 Page seeded by OCA on Sun May 4 01:57:21 2008
Categories: Protein complex | Thermus thermophilus | Fucini, P. | Hanawa-Suetsugu, K. | Harms, J M. | Kaminishi, T. | Kawazoe, M. | RSGI, RIKEN Structural Genomics/Proteomics Initiative. | Schluenzen, F. | Shirouzu, M. | Takemoto, C. | Wilson, D N. | Yokoyama, S. | 30s ribosomal subunit | 5' untranslated region | Mrna capture | National project on protein structural and functional analyse | Nppsfa | Riken structural genomics/proteomics initiative | Rsgi | Shine-dalgarno interaction | Structural genomic | Translation initiation
