2hzd
From Proteopedia
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NMR structure of the DNA-binding TEA domain and insights into TEF-1 function
Contents |
Overview
Transcription enhancer factor 1 is essential for cardiac, skeletal, and, smooth muscle development and uses its N-terminal TEA domain (TEAD) to, bind M-CAT elements. Here, we present the first structure of TEAD and show, that it is a three-helix bundle with a homeodomain fold. Structural data, reveal how TEAD binds DNA. Using structure-function correlations, we find, that the L1 loop is essential for cooperative loading of TEAD molecules on, to tandemly duplicated M-CAT sites. Furthermore, using a microarray, chip-based assay, we establish that known binding sites of the full-length, protein are only a subset of DNA elements recognized by TEAD. Our results, provide a model for understanding the regulation of genome-wide gene, expression during development by TEA/ATTS family of transcription factors.
Disease
Known disease associated with this structure: Sveinsson choreoretinal atrophy OMIM:[189967]
About this Structure
2HZD is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Insights into transcription enhancer factor 1 (TEF-1) activity from the solution structure of the TEA domain., Anbanandam A, Albarado DC, Nguyen CT, Halder G, Gao X, Veeraraghavan S, Proc Natl Acad Sci U S A. 2006 Nov 14;103(46):17225-30. Epub 2006 Nov 3. PMID:17085591
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